Safety Evaluation of Rw after Nasal Inhalation in Rats

Asbestos is reported to cause pulmonary fibrosis, and its use has been banned. We examined the biopersistence and histopathological effect of rock wool (RW) fibers in rat lungs by a nose-only inhalation exposure study. Twenty-four rats were exposed to RW fibers for 6 h daily for 5 consecutive days. Six rats each were sacrificed shortly and 1, 2, and 4 wk after exposure, and the fiber numbers and sizes in lungs were determined. The fiber number in the lungs 4 wk after exposure significantly decreased. The clearance half time was 28 d for fibers with L>20 μm and 50 d for World Health Organization fibers. The reasons for the decrease in number and size of fibers include: 1) discharge outside of the body by mucociliary movement, 2) dissolution by body fluid, and 3) phagocytosis and digestion by alveolar macrophages. Twelve rats were used for histopathological examination, and the pathological changes were classified by Wagner’s criteria. As a result, changes up to only Grade 2 were observed. The reason for the increase in macrophage number was considered to be a transient reaction of body defense. These results suggest that RW has low biopersistence and only a limited patho-